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- Title
Rhabdomere biogenesis in Drosophila photoreceptors is acutely sensitive to phosphatidic acid levels.
- Authors
Raghu, Padinjat; Coessens, Elise; Manifava, Maria; Georgiev, Plamen; Pettitt, Trevor; Wood, Eleanor; Garcia-Murillas, Isaac; Okkenhaug, Hanneke; Trivedi, Deepti; Qifeng Zhang; Razzaq, Azam; Zaid, Ola; Wakelam, Michael; O'Kane, Cahir J.; Ktistakis, Nicholas
- Abstract
Phosphatidic acid (PA) is postulated to have both structural and signaling functions during membrane dynamics in animal cells. In this study, we show that before a critical time period during rhabdomere biogenesis in Drosophila melanogasterphotoreceptors, elevated levels of PA disrupt membrane transport to the apical domain. Lipidomic analysis shows that this effect is associated with an increase in the abundance of a single, relatively minor molecular species of PA. These transport defects are dependent on the activation state of Arf1. Transport defects via PA generated by phospholipase D require the activity of type I phosphatidylinositol (PI) 4 phosphate 5 kinase, are phenocopied by knockdown of P14 kinase, and are associated with normal endoplasmic reticulum to Golgi transport. We propose that PA levels are critical for apical membrane transport events required for rhabdomere biogenesis.
- Subjects
PHOSPHATIDIC acids; CELL membrane formation; DROSOPHILA melanogaster; PHOTORECEPTORS; BIOLOGICAL transport; GOLGI apparatus
- Publication
Journal of Cell Biology, 2009, Vol 185, Issue 1, p129
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200807027