We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Imipenem Resistance Mediated by bla OXA-913 Gene in Pseudomonas aeruginosa.
- Authors
Moon, Dong-Chan; Mechesso, Abraham Fikru; Kang, Hee-Young; Kim, Su-Jeong; Choi, Ji-Hyun; Song, Hyun-Ju; Yoon, Soon-Seek; Lim, Suk-Kyung
- Abstract
Treatment of infectious diseases caused by carbapenem-resistant Pseudomonas aeruginosa is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in P. aeruginosa isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute recommendations. We performed polymerase chain reaction and whole-genome sequencing to detect carbapenem resistance genes. Genomic DNA of P. aeruginosa K19PSE24 was sequenced via the combined analysis of 20-kb PacBio SMRTbell and PacBio RS II. Peptide-Peptide Nucleic Acid conjugates (P-PNAs) targeting the translation initiation region of blaOXA-913 were synthesized. The isolate (K19PSE24) was resistant to imipenem and piperacillin/tazobactam yet was susceptible to most of the tested antimicrobials. Whole-genome sequencing revealed that the K19PSE24 genome comprised a single contig amounting to 6,815,777 base pairs, with 65 tRNA and 12 rRNA genes. K19PSE24 belonged to sequence type 313 and carried the genes aph(3)-IIb, fosA, catB7, crpP, and blaOXA-913 (an allele deposited in GenBank but not described in the literature). K19PSE24 also carried genes encoding for virulence factors (exoenzyme T, exotoxin A, and elastase B) that are associated with adhesion, invasion, and tissue lysis. Nevertheless, we did not detect any of the previously reported carbapenem resistance genes. This is the first report of the blaOXA-913 gene in imipenem-resistant P. aeruginosa in the literature. Notably, no viable colonies were found after co-treatment with imipenem (2 µg/mL) and either of the P-PNAs (12.5 µM or 25 µM). The imipenem resistance in K19PSE24 was primarily due to blaOXA-913 gene carriage.
- Subjects
PSEUDOMONAS aeruginosa; CARBAPENEM-resistant bacteria; IMIPENEM; ACINETOBACTER baumannii; BASE pairs; NUCLEOTIDE sequencing; PSEUDOMONAS syringae
- Publication
Antibiotics (2079-6382), 2021, Vol 10, Issue 10, p1188
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics10101188