We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of stable isotope dilution assays for the quantitation of the food odorants hydrogen sulphide, methanethiol, ethanethiol, and propane-1-thiol and application to durian ( Durio zibethinus L.) pulp.
- Authors
Li, Jia-Xiao; Schieberle, Peter; Steinhaus, Martin
- Abstract
Stable isotope dilution assays were developed for the quantitation of the highly volatile odorants hydrogen sulphide, methanethiol, ethanethiol, and propane-1-thiol using sodium (S)sulphide, sodium (H)methanethiolate, (H)ethanethiol, and (H)propane-1-thiol as internal standards and 3-buten-2-one as derivatisation reagent. Applying sodium as the reducing agent, sodium (S)sulphide was synthesised from S and sodium (H)methanethiolate from (H)dimethyl sulphide. The analytical approach included homogenisation of the food material with the added standards, derivatisation, solvent extraction, and solvent-assisted flavour evaporation (SAFE). Separate detection of analytes and standards was achieved by two-dimensional heart-cut gas chromatography-mass spectrometry using chemical ionisation and characteristic ions of the derivatives 4,4′-sulphanediyldi-2-butanone, 4-(methylsulphanyl)butan-2-one, 4-(ethylsulphanyl)butan-2-one, and 4-(propylsulphanyl)butan-2-one and their isotopically substituted analogues. Application of the approach to durian pulp resulted in high odour activity values (OAV = concentration/odour threshold) with ethanethiol (OAV 480000) being the most potent odorant, followed by methanethiol (OAV 45000), propane-1-thiol (OAV 6300), and hydrogen sulphide (OAV 330).
- Subjects
ISOTOPE dilution analysis; STABLE isotopes; FOOD aroma; HYDROGEN sulfide; METHANETHIOL; ETHANETHIOL; PROPANE; DURIAN
- Publication
European Food Research & Technology, 2017, Vol 243, Issue 1, p69
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-016-2723-z