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- Title
Performance of pseudo‐specific cryogel in lysozyme purification from chicken egg white.
- Authors
Meira, Ana Cristina Freitas de Oliveira; da Silva, Richard Marins; Neves, Isabelle Cristina Oliveira; Minim, Luis Antônio; Veríssimo, Lizzy Ayra Alcântara; de Resende, Jaime Vilela
- Abstract
The application of cryogels for biomolecule purification has expanded due to their adsorption efficiency and operational advantages. In this study, polyacrylamide cryogels functionalized with l‐phenylalanine (cryogel‐Phe) via the glutaraldehyde method were designed for lysozyme adsorption. Cryogel functionalization was confirmed by Fourier‐transform infrared spectroscopy and Kjeldahl analysis, indicating the immobilization of 458.65 mgphenylalanine gcryogel−1. Cryogel‐Phe showed high porosity (0.95) and a Young's modulus of 526.71 kPa. Thermogravimetric analysis indicated that thermal degradation occurred above 200°C. Differential scanning calorimetry and X‐ray diffraction confirmed that the cryogel material was amorphous. In addition, the column presented a hydraulic permeability of 4.15 × 10−13 m2, axial dispersion ranging from 10−7 to 10−6 m2 s−1, and a height equivalent to a theoretical plate ranging from 0.10 to 0.21 cm. The highest adsorption of lysozyme (67.65 mg g−1) was obtained using sodium thiocyanate saline solution (0.025 mol L−1, pH 5.0). The ability of the cryogel‐Phe column to capture and purify lysozyme was confirmed by high enzymatic activity (1294.17 U ml−1), purity (87.92%), purification factor (11.49), and sulphate‐polyacrylamide electrophoresis gel (SDS‐PAGE) electrophoresis gel.
- Subjects
POLYACRYLAMIDE gel electrophoresis; EGGS; LYSOZYMES; EGG whites; YOUNG'S modulus; DIFFERENTIAL scanning calorimetry
- Publication
Canadian Journal of Chemical Engineering, 2023, Vol 101, Issue 6, p3497
- ISSN
0008-4034
- Publication type
Article
- DOI
10.1002/cjce.24703