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- Title
Genetic diversity of Booroola gene in Northeast Bulgarian Merino sheep breed.
- Authors
Bozhilova-Sakova, Milena; Dimitrova, Ivona; Ignatova, Maya
- Abstract
The BMPR-1B/FecB or Booroola gene is the first major fecundity gene identified in sheep. The present investigation was carried out to study polymorphism by PCRRFLP of FecB gene in 30 ewes belonging to the Northeast Bulgarian Merino sheep breed. Genomic DNA was extracted from whole blood. PCR amplification of FecB gene revealed fragments with length 190 bp using a specific primer set. After PCRRFLP analysis it was found the wild allele "+" and the mutant allele "B" with frequencies 0.97 and 0.03, respectively. This is the first study in Bulgarian sheep breeds, where was found the presence of Booroola mutation. According to the results the genotype frequencies were as followed: wild homozygous genotype "++" 0.93 and heterozygous genotype "B+" 0.07, respectively. The observed (Ho) and expected heterozygosity (He) were 0.066 and 0.058, respectively. It can be concluded that the studied herd was in Hardy-Weinberg equilibrium. PCR-RFLP technique can be used to detect and genotype the FecB gene clearly.
- Subjects
SHEEP breeds; NUCLEIC acid isolation methods; POLYMERASE chain reaction
- Publication
Journal of BioScience & Biotechnology, 2020, Vol 9, Issue 2, p19
- ISSN
1314-6238
- Publication type
Article