We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
T-cell antigenic determinants within hepatitis C virus nonstructural protein 3 and cytokine production profiles in hepatitis C.
- Authors
Pan, C.-H.; Yang, P.-M.; Hwang, L.-H.; Kao, Shing-F.; Chen, P.-J.; Chiang, B.-L.; Chen, D.-S.
- Abstract
summary. The aim of this study was to further investigate the role of T-helper cells in hepatitis C virus (HCV) infection, focusing on the T-cell antigenic determinants and cytokine profiles of nonstructural 3 (NS3) protein-stimulated peripheral blood mononuclear cells (PBMCs) of HCV patients. A total of 12 recombinant proteins of theNS3 region were purified and used to test T-cell proliferative response and antigenic determinants of HCV-seropositive patients. In addition, cytokines produced by antigen stimulated PBMCs were measured. Our data showed that PBMCs from 55.7% (34/61) of HCV patients proliferated to at least one antigen, but PBMCs of HCV seronegative patients did not. In addition, PBMCs from about 82.0% (32/39) HCV-seropositive patients produced significant amounts of cytokines (10 pg/mL). Interestingly, PBMCs from 66% of patients produced TH2 -related cytokines such as interleukin (IL)-4 and IL-5. In mappingexperiments, the data showed multiple T-cell antigenic determinants. Our data demonstrated that NS3 antigen-stimulated PBMCs of HCV patients recognized multiple T-cell antigenic determinants and produced significant amounts of TH0 or TH2 -related cytokines, which might play a critical role in the chronicity of HCV infection.
- Subjects
HEPATITIS C; T cells; CYTOKINES
- Publication
Journal of Viral Hepatitis, 2002, Vol 9, Issue 4, p258
- ISSN
1352-0504
- Publication type
Article
- DOI
10.1046/j.1365-2893.2002.00363.x