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- Title
Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses.
- Authors
Jiangqin Zhao; Ragupathy, Viswanath; Jikun Liu; Xue Wang; Sai Vikram Vemula; Haja Sittana El Mubarak; Zhiping Ye; Landry, Marie L.; Hewlett, Indira
- Abstract
Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics.
- Subjects
INFLUENZA viruses; NUCLEOTIDE sequence; PANDEMICS; VIRUS diseases; INFECTION
- Publication
Emerging Infectious Diseases, 2015, Vol 21, Issue 3, p400
- ISSN
1080-6040
- Publication type
Article
- DOI
10.3201/eid2103.141169