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- Title
Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery.
- Authors
Golzio, M.; Mazzolini, L.; Moller, P.; Rols, M. P.; Teissié, J.
- Abstract
Owing to their capacity to induce strong, sequence-specific, gene silencing in cells, short interfering RNAs (siRNAs) represent new potential therapeutic tools. This development requires, however, new safe and efficient in vivo siRNA delivery methods. In the present technical report, we show that electrically mediated siRNA transfer can suppress transgene expression in adult mice muscles. Using electropulsation for siRNA delivery opens the way for a targeted gene silencing on a broad range of tissues. Clinical applications of electropulsation for delivery of other classes of molecules are under trials. We reported that gene silencing was efficiently obtained in vivo in an adult mammal (mouse) with chemically synthesized siRNA after its electrical delivery. The associated gene silencing was followed on the same animal and lasted at least 11 days. Gene silencing was obtained in muscles not only on young adult mice but also on much older animals. No tissue damages were detected under our electrical conditions. Therefore, this method should provide an efficient approach for a localized delivery of siRNAs in various tissues and organs.Gene Therapy (2005) 12, 246-251. doi:10.1038/sj.gt.3302405 Published online 9 December 2004
- Subjects
GENE silencing; ORGANS (Anatomy); CRYOBIOLOGY; HEALTH care rationing; TRANSGENE expression; YOUNG adults
- Publication
Gene Therapy, 2005, Vol 12, Issue 3, p246
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3302405