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- Title
Hominis placenta Suppresses Acute Lung Inflammation by Activating Nrf2.
- Authors
Kim, Tae Ho; Choi, Jun-Yong; Kim, Kyun Ha; Kwun, Min Jung; Han, Chang-Woo; Won, Ran; Lee, Jung Ju; Kim, Jong-In; Joo, Myungsoo
- Abstract
Hominis placenta (HP), a dried human placenta, has been known to target liver, lung, or kidney meridians, improving the functions associated with these meridians in traditional Chinese or Asian medicine (TCM). Since recent studies implicate an HP extract in suppressing inflammation, we investigated whether an aqueous HP extract can ameliorate inflammation that occurred in the lungs. When administered with a single intratracheal lipopolysaccharide (LPS), C57BL/6 mice developed an acute neutrophilic lung inflammation along with an increased expression of pro-inflammatory cytokine genes. However, this was diminished by the administration HP extract via an intraperitoneal route 2 h after LPS treatment. Western blot and semi-quantitative RT-PCR analyses revealed that while suppressing the activity of a proinflammatory factor NF-κB marginally, the HP extract strongly activated an anti-inflammatory factor Nrf2, with concomitant expression of Nrf2-dependent genes. Mechanistically, the HP extract suppressed the ubiquitin-mediated degradation of Nrf2, functioning similarly to a 26S proteasome inhibitor, MG132. Collectively, these results suggest that the HP extract suppresses inflammation in mouse lungs, which is in part related to the HP extract perturbing the ubiquitin-dependent degradation of Nrf2 and thus increasing the function of Nrf2.
- Subjects
ANIMAL experimentation; INFLAMMATION; LUNG injuries; CHINESE medicine; MICE; PLACENTA; POLYMERASE chain reaction; RESEARCH funding; T-test (Statistics); WESTERN immunoblotting; ACUTE diseases; ONE-way analysis of variance
- Publication
American Journal of Chinese Medicine, 2018, Vol 46, Issue 4, p801
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X18500428