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- Title
Effects of nitrosyl complexes of iron with functional S-ligands on the activity of hydrolytic enzymes.
- Authors
Tat'yanenko, L.; Dobrokhotova, O.; Pikhteleva, I.; Poletaeva, D.; Kotel'nikov, A.; Rudneva, T.; Sanina, N.; Aldoshin, S.
- Abstract
We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe(SR′)(NO)] with R′ = cysteamine and penicillamine - and of the neutral type - [Fe(SR″)(NO)] with R″ = benzthiazoline - on the enzymatic activity of hydrolases - cyclic guanosine monophosphate phosphodiesterase (PDEcGMP) and sarcoplasmic reticulum Ca-Mg-dependent ATPase (SR-Ca-ATPase). All the complexes studied were found to be modulators of both enzymes. They produced weak inhibition of PDEcGMP activity and marked inhibition of active transport by SR Ca-ATPase. While having virtually no effect on the hydrolytic center of SR Ca-ATPase, the active transport of calcium was completely blocked over the concentration range 1 μM - 0.1 mM, with uncoupling of the hydrolytic and transport functions of this enzyme. This suggests that these complexes can induce structural-functional changes in SR Ca-ATPase at concentrations corresponding to an enzyme:inhibitor ratio of 1:1, which is consistent with the antimetastatic action of these compounds.
- Subjects
METAL complexes; NITROSYL compounds; ORGANOIRON compounds; LIGANDS (Biochemistry); HYDROLASES; CYSTEAMINE; PENICILLAMINE; GUANOSINE monophosphate phosphodiesterase
- Publication
Pharmaceutical Chemistry Journal, 2012, Vol 45, Issue 11, p651
- ISSN
0091-150X
- Publication type
Article
- DOI
10.1007/s11094-012-0696-1