We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Advanced optical microscopy methods for in vivo imaging of sub-cellular structures in thick biological tissues.
- Authors
Chen, Nanguang; Rehman, Shakil; Sheppard, Colin J. R.
- Abstract
Optical microscopy has become an indispensable tool for visualizing sub-cellular structures and biological processes. However, scattering in biological tissues is a major obstacle that prevents high-resolution images from being obtained from deep regions of tissue. We review common techniques, such as multiphoton microscopy (MPM) and optical coherence microscopy (OCM), for diffraction limited imaging beyond an imaging depth of 0.5 mm. Novel implementations have been emerging in recent years giving higher imaging speed, deeper penetration, and better image quality. Focal modulation microscopy (FMM) is a novel method that combines confocal spatial filtering with focal modulation to reject out-of-focus background. FMM has demonstrated an imaging depth comparable to those of MPM and OCM, near-real-time image acquisition, and the capability for multiple contrast mechanisms.
- Subjects
MICROSCOPY; HIGH resolution imaging; IMAGE quality analysis; MICRO-optics; MICROMETRY; BEAMFORMING; SCATTERING (Physics)
- Publication
Journal of Innovative Optical Health Sciences, 2014, Vol 7, Issue 5, p-1
- ISSN
1793-5458
- Publication type
Article
- DOI
10.1142/S179354581440001X