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- Title
IN VITRO EVALUATION OF NATURAL AND SYNTHETIC SUBSTRATE FOR BIOFILM FORMATION AND THEIR EFFECT ON WATER QUALITIES.
- Authors
Pandey, P. K.; Laxmi, M. S.; Kumar, Saurav
- Abstract
The present study evaluated the natural and synthetic substrate for biofilm formation in in vitro condition and their effect on physico-chemical parameters of water. The four different treatments i.e., control (without substrate), T1 (sugarcane bagasse), T2 (PVC), T3 (coconut husk) and T4 (bamboo strips) as substrates were used for assessment of biofilm formation and heterotrophic bacterial growth. Physico-chemical parameters of water and total platecolonies were estimated at weekly intervals for five weeks. Results of dissolved oxygen, total alkalinity, as well as nutrients like ammonia-nitrogen, nitrate-nitrogen and nitrite-nitrogen showed significant (p< 0.05) difference in the treatment groups compared with control. The total bacterial count of the substrata significantly (p< 0.05) high in all the treatment groups during the 3rd week and the highest value was recorded in the treatment T3 (12.42 × 103cfucm-2). Based on the present in vitro study, it may be concluded that biodegradable substrates especially sugarcane bagasse significantly enhanced the physico-chemical parameters of waters and optimized the favourable conditions for heterotrophic bacterial growth in biofilm, making it the most suitable substrate which can be used in aquaculture system as production enhancement strategy.
- Subjects
BIOFILMS -- Environmental aspects; WATER quality; HETEROTROPHIC bacteria; AQUATIC organisms; POLYVINYL chloride; BIOCHEMICAL substrates; AUTOTROPHIC bacteria
- Publication
Indian Journal of Animal Research, 2014, Vol 48, Issue 6, p585
- ISSN
0367-6722
- Publication type
Article
- DOI
10.5958/0976-0555.2014.00036.3