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- Title
Proteomics‐based milk whey proteome profiling of Indian Jersey crossbreed cows followed by chromosomal mapping.
- Authors
Singh, Manish Kumar; Kumar, Arvind; Nimmanapalli, Ramadevi; Pandey, Anand Kumar
- Abstract
BACKGROUND: Milk contains a massive class of minor proteins that are known for their various biological and molecular functions. Many whey proteins transfer the host defense mechanism to the human body. In this assay, electrophoresis followed by a high‐resolution mass spectrometry‐based proteomic approach has been applied to identify the whey proteome of Indian Jersey crossbreed bovines. RESULTS: Two search engines, MS Amanda and Sequest HT, have shown more than 29 minor proteins. Chromosomal mapping revealed that chromosomes 5 and 9 are expressing maximum proteins in the whey proteome. The principal component analysis, outlier plots, scree plots, score plots, and loading plots were generated to further assess the results. CONCLUSION: The majorly expressed ones are glycosylation‐dependent cell adhesion molecule‐1, ubiquitin, desmoglein, annexin, glycoprotein, arginase, histones, peroxiredoxin, vimentin, desmin, catenin, peripherin, and 70 kDa heat shock protein. © 2023 Society of Chemical Industry.
- Subjects
SOCIETY of Chemical Industry (Great Britain); HEAT shock proteins; MICROSOFT Corp.; WHEY; DESMOGLEINS; WHEY proteins; MILK proteins; PRINCIPAL components analysis; UBIQUITIN
- Publication
Journal of the Science of Food & Agriculture, 2023, Vol 103, Issue 11, p5634
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.12640