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- Title
In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts.
- Authors
Kazeem, Mutiu Idowu; Ogungbe, Semawon Mauton; Saibu, Gbemisola Morounke; Aboyade, Oluwaseyi Mayode
- Abstract
Inhibition of some carbohydrate metabolizing enzymes is one of the modes of action of antihyperglycemic agents. The aim of this study was to investigate the in vitro inhibition of α-amylase and α-glucosidase by extracts of N. tabacum leaf. Powdered leaves were extracted with acetone, ethanol and water, and tested for their ability to inhibit α-amylase from Aspergilus oryzae and α- glucosidase from Saccharomyces cerevisae. The results revealed that aqueous extract of the plant was most effective inhibitor of α-amylase (IC50 5.70 mg/ mL) while acetone extract exhibited the best inhibitory potential on α-glucosidase (IC50 4.50 mg/mL). Kinetic studies showed that the mode of inhibition of α-amylase by aqueous extract was non-competitive while that of the acetone extract on α-glucosidase was competitive. The observed inhibitions of α-amylase and α-glucosidase suggest that the leaf extracts of N. tabacum may be useful in the management of Diabetes mellitus, which may due to the presence of phytochemicals.
- Subjects
TOBACCO; PLANT extracts; HYPOGLYCEMIC agents; CARBOHYDRATE metabolism; AMYLASE inhibitors; GLUCOSIDASE inhibitors; SACCHAROMYCES cerevisiae
- Publication
Bangladesh Journal of Pharmacology, 2014, Vol 9, Issue 2, p140
- ISSN
1991-007X
- Publication type
Article
- DOI
10.3329/bjp.v9i2.17540