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- Title
Alkaloid biosynthesis and metabolic profiling responses to jasmonic acid elicitation in Hamelia patens plants by NMR-based metabolomics.
- Authors
Flores-Sanchez, Isvett; Paniagua-Vega, David; Vera-Reyes, Ileana; Cerda-García-Rojas, Carlos; Ramos-Valdivia, Ana
- Abstract
Introduction: Hamelia patens is a perennial shrub native from Mexico which is used in traditional medicine to treat chronic wounds and tumors. Bioactive monoterpenoid indole alkaloids (MIAs) and monoterpenoid oxindole alkaloids (MOAs) have been isolated from this species, associated with the ethnomedical use. Objectives: In order to study the biosynthesis and production of these alkaloids, H. patens plants were treated with the stress hormone jasmonic acid. Methods: Nuclear magnetic resonance-based metabolic profiles and multivariate data analysis were applied to explore changes or variability in elicited and control plants. Results: According to the partial least square-discriminant analysis from MeOH/HO fractions, sucrose, tryptophan, glutamic acid, glutamine, strictosidine, p-coumaric acid, chlorogenic acid, and the MOAs isopteropodine, palmirine and pteropodine were predominant in the treated plants, while glucose, aspartic acid, acetic acid, and loganic acid concentrations were higher in control plants. HPLC analysis showed that accumulation of pteropodine, isopteropodine, speciophylline, rumberine, hameline and palmirine in elicited plants was preceded by an increase in the transcription level of 1-deoxy- d-xylulose-5-phosphate synthase (DXS, EC 2.2.1.7) and strictosidine synthase (STR, EC 4.3.3.2), and STR activity during a time course. However, no inductions of transcription and enzyme activity of strictosidine β-glucosidase (SGD, EC 3.2.1.105) were observed in elicited plants. Conclusion: Correlations between primary and secondary metabolism biosynthetic pathways, such as the alkaloid and chlorogenic acid biosynthesis were found. Also, their implications in the plant fitness to stress are proposed. Confirmation of tryptophan as a precursor of MIA and MOA, via shikimate pathway, was done by L-[3′-C]-tryptophan feeding.
- Subjects
ALKALOID synthesis; PERENNIALS; TRADITIONAL medicine; CHRONIC wounds &; injuries; BIOSYNTHESIS; THERAPEUTICS
- Publication
Metabolomics, 2016, Vol 12, Issue 4, p1
- ISSN
1573-3882
- Publication type
Article
- DOI
10.1007/s11306-016-0999-4