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- Title
Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study.
- Authors
Sharma, Rama; Reddy, Vamsi Krishna L.; Prashant, G. M.; Ojha, Vivek; Kumar, Naveen P. G.
- Abstract
Context: Several studies have demonstrated the activity of natural plants on the dental biofi lm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it. Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Streptococcus mutans to glass surface. Materials and Methods: Test solutions were prepared. For antimicrobial activity testing, tubes containing test solution and culture medium were inoculated with a suspension of S. mutans followed by plating on Brain Heart Infusion (BHI) agar. S. mutans adherence to glass in presence of the different test solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method. Statistical Analysis: Statistical signifi cance was measured using one way ANOVA followed by Tukey's post hoc test. P value < 0.05 was considered statistically signifi cant. Results: Pure chicory had shown signifi cantly less bacterial count compared to all other groups. Groups IV and V had shown signifi cant reduction in bacterial counts over the period of 4 hrs. Regarding anti-adherence effect, group I-IV had shown signifi cantly less adherence of bacteria to glass surface. Conclusions: Chicory exerted antibacterial effect against S. mutans while coffee reduced signifi cantly the adherence of S. mutans to the glass surface.
- Subjects
STREPTOCOCCUS mutans; DENTAL caries; CHICORY; COFFEE; ANTI-infective agents
- Publication
Journal of Oral & Maxillofacial Pathology (0973029X), 2014, Vol 18, Issue 2, p201
- ISSN
0973-029X
- Publication type
Article
- DOI
10.4103/0973-029X.140749