We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Investigation of cytokinin-deficient phenotypes in Arabidopsis by ectopic expression of orchid DSCKX1
- Authors
Yang, Shuhua; Yu, Hao; Xu, Yifeng; Goh, Chong Jin
- Abstract
The plant hormone cytokinin plays a major role in regulating plant growth and development. Here we generated cytokinin-reduction Arabidopsis plants by overexpressing a heterologous cytokinin oxidase gene DSCKX1 from Dendrobium orchid. These transgenic plants exhibited reduced biomass, rapid root growth, decreased ability to form roots in vitro, and reduced response to cytokinin in growing calli and roots. Furthermore, the expression of KNAT1, STM, and CycD3 genes was significantly reduced in the transgenic plants, suggesting that cytokinin may function to control the cell cycles and shoot/root development via regulation of these genes.
- Subjects
PLANT hormones; CYTOKININS; PLANT growth; DENDROBIUM
- Publication
FEBS Letters, 2003, Vol 555, Issue 2, p291
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01259-6