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- Title
Isolation and amplification of genomic DNA from nutmeg mace.
- Authors
Swetha, V. P.; Parvathy, V. A.; Sheeja, T. E.; Sasikumar, B.
- Abstract
A reliable and efficient protocol for isolation and amplification of genomic DNA from dried mace of Myristica fragrans, was developed. The yield of genomic DNA was 231.4 µg g-1 and 306.8 µg g-1, respectively for the samples procured from the farm and market. The absorbance ratio at A260/A280 was greater than 1.8 indicating the good quality of DNA. Complete restriction digestion and PCR amplification of genomic DNA further confirmed the quality of isolated DNA.
- Subjects
NUCLEIC acid isolation methods; SALT; LIGHT absorbance; BIODEGRADATION; METABOLITES; POLYSACCHARIDES
- Publication
Indian Journal of Biotechnology, 2018, Vol 17, Issue 3, p533
- ISSN
0972-5849
- Publication type
Article