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- Title
Glucose-Dependent Modulation of Insulin Secretion and Intracellular Calcium Ions by GKA50, a Glucokinase Activator.
- Authors
Johnson, Daniel; Shepherd, Ruth M.; Gill, Debra; Gorman, Tracy; Smith, David M.; Dunne, Mark J.
- Abstract
Because glucokinase is a metabolic sensor involved in the regulated release of insulin, we have investigated the acute actions of novel glucokinase activator compound 50 (GKA50) on islet function. Insulin secretion was determined by enzyme-linked immunosorbent assay, and microfluorimetry with fura-2 was used to examine intracellular Ca2+ homeostasis ([Ca2+]i) in isolated mouse, rat, and human islets of Langerhans and in the MIN6 insulin-secreting mouse cell line. In rodent islets and MIN6 cells, 1 µmol/l GKA50 was found to stimulate insulin secretion and raise [Ca2+]i in the presence of glucose (2-10 mmol/l). Similar effects on insulin release were also seen in isolated human islets. GKA50 (1 µmol/l) caused a leftward shift in the glucose-concentration response profiles, and the half-maximal effective concentration (EC50) values for glucose were shifted by 3 mmol/l in rat islets and ∼10 mmol/l in MIN6 ceils. There was no significant effect of GKA50 on the maximal rates of glucose-stimulated insulin secretion. In the absence of glucose, GKA50 failed to elevate [Ca2+]i (1 µmol/l GKA50) or to stimulate insulin release (30 nmol/l-10 µmol/l GKA50). At 5 mmol/l glucose, the EC50 for GKA50 in MIN6 ceils was ∼0.3 µmol/l. Inhibition of glucokinase with mannoheptulose or 5-thioglucose selectively inhibited the action of GKA50 on insulin release but not the effects of tolbutamide. Similarly 3-methoxyglucose prevented GKA50-induced rises in [Ca2+]i but not the actions of tolbutamide. Finally, the ATP-sensitive K+ channel agonist diazoxide (200 µmol/l) inhibited GKA50-induced insulin release and its elevation of [Ca2+]i We show that GKA50 is a glucose-like activator of β-cell metabolism in rodent and human islets and a Ca2+-dependent modulator of insulin secretion. Diabetes 56:1694-1702, 2007
- Subjects
DIABETES; GLUCOKINASE; ENZYME-linked immunosorbent assay; GLUCOSE; INSULIN; TOLBUTAMIDE; RESPONSE inhibition; CELL lines
- Publication
Diabetes, 2007, Vol 56, Issue 6, p1694
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/db07-0026