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- Title
Producing and Testing Prototype Tissue-Engineered 3D Tri-Leaflet Valved Stents on Biodegradable Poly-ε-Caprolactone Scaffolds.
- Authors
Lutter, Georg; Pommert, Nina Sophie; Zhang, Xiling; Seiler, Jette; Saeid Nia, Monireh; Meier, David; Sellers, Stephanie L.; Gorb, Stanislav N.; Hansen, Jan-Hinnerk; Seoudy, Hatim; Müller, Oliver J.; Saad, Mohammed; Haneya, Assad; Frank, Derk; Puehler, Thomas; Sathananthan, Janarthanan
- Abstract
Transcatheter pulmonary valve replacement is a minimally-invasive alternative treatment for right ventricular outflow tract dysfunction and has been rapidly evolving over the past years. Heart valve prostheses currently available still have major limitations. Therefore, one of the significant challenges for the future is the roll out of transcatheter tissue engineered pulmonary valve replacement to more patients. In the present study, biodegradable poly-ε-caprolactone (PCL) nanofiber scaffolds in the form of a 3D leaflet matrix were successfully seeded with human endothelial colony-forming cells (ECFCs), human induced pluripotent stem cell-derived MSCs (hMSCs), and porcine MSCs (pMSCs) for three weeks for the generation of 3D tissue-engineered tri-leaflet valved stent grafts. The cell adhesion, proliferation, and distribution of these 3D heart leaflets was analyzed using fluorescence microscopy and scanning electron microscopy (SEM). All cell lineages were able to increase the overgrown leaflet area within the three-week timeframe. While hMSCs showed a consistent growth rate over the course of three weeks, ECFSs showed almost no increase between days 7 and 14 until a growth spurt appeared between days 14 and 21. More than 90% of heart valve leaflets were covered with cells after the full three-week culturing cycle in nearly all leaflet areas, regardless of which cell type was used. This study shows that seeded biodegradable PCL nanofiber scaffolds incorporated in nitinol or biodegradable stents will offer a new therapeutic option in the future.
- Subjects
PROSTHETIC heart valves; PULMONARY valve; MECHANICAL hearts; HEART valves; NANOFIBERS; SCANNING electron microscopy; FLUORESCENCE microscopy
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 24, p17357
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms242417357