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- Title
In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes.
- Authors
EbrahimAmini, Azin; Mylvaganam, Shanthini; Bazzigaluppi, Paolo; Khazaei, Mohamad; Velumian, Alexander; Stefanovic, Bojana; Carlen, Peter L.
- Abstract
A normally functioning nervous system requires normal extracellular potassium ion concentration ([K]o). Throughout the nervous system, several processes, including those of an astrocytic nature, are involved in [K]o regulation. In this study we investigated the effect of astrocytic photostimulation on [K]o. We hypothesized that in vivo photostimulation of eNpHR-expressing astrocytes leads to a decreased [K]o. Using optogenetic and electrophysiological techniques we showed that stimulation of eNpHR-expressing astrocytes resulted in a significantly decreased resting [K]o and evoked K responses. The amplitude of the concomitant spreading depolarization-like events also decreased. Our results imply that astrocytic membrane potential modification could be a potential tool for adjusting the [K]o.
- Subjects
ASTROCYTES; POTASSIUM ions; NERVOUS system; MEMBRANE potential
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 16, p8658
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22168658