We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman.
- Authors
Aguiar, Jaciana S.; Araújo, Rosilma O.; Rodrigues, Maria do Desterro; Sena, Kêsia X. F. R.; Batista, André M.; Guerra, Maria M. P.; Oliveira, Steno L.; Tavares, Josean F.; Silva, Marcelo S.; Nascimento, Silene C.; da Silva, Teresinha Gonçalves
- Abstract
In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6ß-Benzoyloxy-3α-(3,4,5- trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 µg/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95:5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95:5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis.
- Subjects
METHANOL; ERYTHROXYLUM; HEMOLYSIS &; hemolysins; ERYTHROCYTES; LABORATORY mice; TROPANES
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 4, p4124
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms13044124