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- Title
Transcription factor and microRNA regulation in androgen-dependent and -independent prostate cancer cells.
- Authors
Guohua Wang; Yadong Wang; Weixing Feng; Xin Wang; Yang, Jack Y.; Yuming Zhao; Yue Wang; Yunlong Liu
- Abstract
Background: Prostate cancer is one of the leading causes of cancer death in men. Androgen ablation, the most commonly-used therapy for progressive prostate cancer, is ineffective once the cancer cells become androgen-independent. The regulatory mechanisms that cause this transition (from androgen-dependent to androgen-independent) remain unknown. In this study, based on the microarray data comparing global gene expression patterns in the prostate tissue between androgen-dependent and -independent prostate cancer patients, we indentify a set of transcription factors and microRNAs that potentially cause such difference, using a model-based computational approach. Results: From 335 position weight matrices in the TRANSFAC database and 564 microRNAs in the microRNA registry, our model identify 5 transcription factors and 7 microRNAs to be potentially responsible for the level of androgen dependency. Of these transcription factors and microRNAs, the estimated function of all the 5 transcription factors are predicted to be inhibiting transcription in androgen-independent samples comparing with the dependent ones. Six out of 7 microRNAs, however, demonstrated stimulatory effects. We also find that the expression levels of three predicted transcription factors, including AP-1, STAT3 (signal transducers and activators of transcription 3), and DBP (albumin D-box) are significantly different between androgen-dependent and -independent patients. In addition, microRNA microarray data from other studies confirm that several predicted microRNAs, including miR-21, miR-135a, and miR-135b, demonstrate differential expression in prostate cancer cells, comparing with normal tissues.
- Subjects
PROSTATE cancer; TRANSCRIPTION factors; CANCER cells; ANDROGENS; CANCER patients; GENE expression
- Publication
BMC Genomics, 2008, Vol 9, p1
- ISSN
1471-2164
- Publication type
Article
- DOI
10.1186/1471-2164-9-S2-S22