We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A (positive) blot on his record.
- Authors
Eisenstein, Michael
- Abstract
Reports on the development of a method used to characterize the two 5S ribosomal RNA genes from Xenopus laevis by Edwin Southern in 1970 in Edinburgh, Scotland. Significance of the study at a time when cloning technologies were not yet widely available and techniques for detecting specific DNA sequences were limited; Sensitivity and specificity of the technique; Success of Southern in developing an alternative strategy that rely on the capillary action of transfer buffer for the upward blotting through of DNA.
- Subjects
EDINBURGH (Scotland); SCOTLAND; PAPER electrophoresis; NUCLEIC acid analysis; XENOPUS laevis; NUCLEOTIDE sequence; CYTOCHEMICAL bioassay; SOUTHERN, Edwin; CAPILLARY electrophoresis
- Publication
Nature Methods, 2004, Vol 1, Issue 1, p90
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1004-90