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- Title
Hydrolysis of [17,17-<sup>2</sup>H<sub>2</sub>]gibberellin A<sub>20</sub>-glucoside and [17,17-<sup>2</sup>H<sub>2</sub>]gibberellin A<sub>20</sub>-glucosyl ester by Azospirillum lipoferum cultured in a nitrogen-free biotin-Based chemically-defined medium
- Authors
Piccoli, Patricia; Lucangeli, Carlos; Bottini, Rubén; Schneider, Gernot
- Abstract
Azospirillum lipoferum strain USA 5b, a gibberellin producing bacterium, was cultured in a nitrogen-free biotin-based chemically-defined medium in the presence of the glucosyl ester or the 13-O-glucoside of [17,17-2H2]-gibberellin A20. The [17,17-2H2]-gibberellin A20 conjugates were added at both the stationary phase of the cultures and at the beginning of the growth curve. Metabolism of the conjugates was examined after 72 h of incubation using capillary gas chromatography-mass spectrometry, with identification by full scan mass spectra. Metabolites identified were [17,17-2H2]-gibberellin A20, [17,17-2H2]-gibberellin A1 and [17,17-2H2]-gibberellin A3. Also, in the Azospirillum cultures fed at the beginning of the growth curve, gibberellin A5 and gibberellin A20 were characterized as endogenous by mass spectrometry/full spectrum. These results support the concept that the growth promotion in plants that is induced by Azospirillum infection may occur by a combination of both gibberellin production and gibberellin-glucoside/glucosyl ester deconjugation by the bacterium.
- Publication
Plant Growth Regulation, 1997, Vol 23, Issue 3, p179
- ISSN
0167-6903
- Publication type
Article
- DOI
10.1023/A:1005925925127