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- Title
Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match.
- Authors
Nielsen, Joachim; Krustrup, Peter; Nybo, Lars; Gunnarsson, Thomas; Madsen, Klavs; Schrøder, Henrik; Bangsbo, Jens; Ørtenblad, Niels
- Abstract
Whole muscle glycogen levels remain low for a prolonged period following a soccer match. The present study was conducted to investigate how this relates to glycogen content and particle size in distinct subcellular localizations. Seven high-level male soccer players had a vastus lateralis muscle biopsy collected immediately after and 24, 48, 72 and 120 h after a competitive soccer match. Transmission electron microscopy was used to estimate the subcellular distribution of glycogen and individual particle size. During the first day of recovery, glycogen content increased by ~60% in all subcellular localizations, but during the subsequent second day of recovery glycogen content located within the myofibrils (Intramyofibrillar glycogen, a minor deposition constituting 10-15% of total glycogen) did not increase further compared with an increase in subsarcolemmal glycogen (−7 vs. +25%, respectively, P = 0.047). Conversely, from the second to the fifth day of recovery, glycogen content increased (53%) within the myofibrils compared to no change in subsarcolemmal or intermyofibrillar glycogen ( P < 0.005). Independent of location, increment in particle size preceded increment in number of particles. Intriguingly, average particle size decreased; however, in the period from 3 to 5 days after the match. These findings suggest that glycogen storage in skeletal muscle is influenced by subcellular localization-specific mechanisms, which account for an increase in number of glycogen particles located within the myofibrils in the period from 2 to 5 days after the soccer match.
- Subjects
SKELETAL muscle physiology; SPORTS physiology; GLYCOGEN; SUBCELLULAR fractionation; SOCCER tournaments; MUSCLE contraction
- Publication
European Journal of Applied Physiology, 2012, Vol 112, Issue 10, p3559
- ISSN
1439-6319
- Publication type
Article
- DOI
10.1007/s00421-012-2341-9