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- Title
Optimization of liquid-liquid extraction of biosurfactants from corn steep liquor.
- Authors
Vecino, X.; Barbosa-Pereira, L.; Devesa-Rey, R.; Cruz, J.; Moldes, A.
- Abstract
In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.
- Subjects
LIQUID-liquid extraction; CHLOROFORM; BIOSURFACTANTS; MICELLES; NANOPARTICLES; LACTIC acid bacteria
- Publication
Bioprocess & Biosystems Engineering, 2015, Vol 38, Issue 9, p1629
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-015-1404-9