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- Title
The first report on the prevalence of pestivirus infection in camels in Sudan.
- Authors
Intisar, Kamil; Ali, Yahia; Khalafalla, AbdelMelik; Mahasin, E.; Amin, Adil; Taha, Khalid
- Abstract
The role of pestivirus particularly bovine viral diarrhea virus (BVDV) in causing respiratory infections in camels was studied in four different localities in Sudan. The evaluation was carried out using ELISA, and positive specimens were further tested using direct fluorescent antibody technique (FAT) and reverse transcriptase polymerase chain reaction (RT-PCR) for confirmation. The overall detected seroprevalence of BVD in camel sera was 84.6% with the highest prevalence in Western Sudan (92.5%) and with most of positives showing 2+ and 3+ titer. Out of 186 lung specimens examined for BVDV antigen, 13 were found positive (7%) with the highest prevalence in Central Sudan. All ELISA-positive specimens were positive using FAT and RT-PCR. To our knowledge, this is the first report for the detection of BVDV antigen and antibodies in camels in Sudan.
- Subjects
SUDAN; BOVINE viral diarrhea virus; CAMELS; ENZYME-linked immunosorbent assay; FLUORESCENT antibody technique; REVERSE transcriptase polymerase chain reaction; SEROPREVALENCE
- Publication
Tropical Animal Health & Production, 2010, Vol 42, Issue 6, p1203
- ISSN
0049-4747
- Publication type
Article
- DOI
10.1007/s11250-010-9549-7