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- Title
Augmented beta cell loss and mitochondrial abnormalities in sucrose-fed GK rats.
- Authors
Hiroki Mizukami; Ryuichi Wada; Motoi Koyama; Teruko Takeo; Sechiko Suga; Makoto Wakui; Soroku Yagihashi; Mizukami, Hiroki; Wada, Ryuichi; Koyama, Motoi; Takeo, Teruko; Suga, Sechiko; Wakui, Makoto; Yagihashi, Soroku
- Abstract
Progressive decline of islet beta cell mass is a hallmark of type 2 diabetes, where nutritional insults are invoked in the pathologic process. Its detailed mechanisms are, however, incompletely understood. We explored the effect of sucrose diet on mitochondria in Goto Kakizaki (GK) rats, a spontaneously diabetic model. Six-week-old male GK rats were given 30% sucrose orally for 2 weeks. Normal Wistar rats fed with sucrose served as controls. Compared to untreated GK rats, sucrose-fed GK rats showed severe degeneration and death of beta cells with disrupted and swollen mitochondria and a greater beta cell loss. Submicroscopic analysis disclosed a smaller mean volume and a greater number of mitochondria in beta cells in GK rats compared to those in Wistar rats. Mitochondria in sucrose-fed GK rats were 2.4-fold greater in mean volume than those in untreated state. Without sucrose feeding, there was no significant difference in mitochondrial membrane potentials (MmPs) of isolated islets between Wistar and GK rats. MmPs were reduced by 44% in sucrose-fed GK rats but not influenced in sucrose-fed Wistar rats. Current results suggest that nutritional insults like sucrose feeding may exert deleterious effects on mitochondria, resulting in augmented beta cell loss in type 2 diabetes.
- Subjects
RATS; BLIND mole rats; BUNOMYS; COTTON rats; MITOCHONDRIAL pathology; BIOLOGICAL models; RESEARCH; BODY weight; BIOLOGICAL transport; ANIMAL experimentation; RESEARCH methodology; DIABETES; BLOOD sugar; MEDICAL cooperation; EVALUATION research; TYPE 2 diabetes; ISLANDS of Langerhans; DIETARY sucrose; MITOCHONDRIA; INSULIN; COMPARATIVE studies; CELL death
- Publication
Virchows Archiv: European Journal of Pathology, 2008, Vol 452, Issue 4, p383
- ISSN
0945-6317
- Publication type
journal article
- DOI
10.1007/s00428-007-0508-2