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- Title
FENOTYPOVÉ A GENOTYPOVÉ CHARAKTERISTIKY ANTIBIOTICKEJ REZISTENCIE U NEMOCNIČNÝCH KMEŇOV KLEBSIELLA PNEUMONIAE V FAKULTNEJ NEMOCNICI S POLIKLINIKOU ŽILINA.
- Authors
Martin, NOVÁK; Katarína, KAŠLÍKOVÁ; Elena, NOVÁKOVÁ; Michala, VLADÁROVÁ; Lucia, SLIVIAKOVÁ; Henrieta, HUDEČKOVÁ
- Abstract
Background: A significant risk in healthcare is posed by the agents of nosocomial infections resistant to antibiotics, including the bacterium Klebsiella pneumoniae. Aims: To determine the sensitivity of samples to selected antibiotics, to prove the presence of carbapenemase in selected samples, to compare the determined sensitivity to antibiotics in samples between the methods used and with respect to the origin of the sample. Research sample and methods: The sample consisted of 54 hospital strains of Klebsiella pneumoniae from the University Hospital with a polyclinic in Žilina, collected in 2020. We used the disk diffusion method and the microdilution method to determine antibiotic susceptibility. We used the polymerase chain reaction to detect carbapenemase. Results: In the disk diffusion test as well as in the microdilution method, the most effective antibiotic was meropenem with a determined sensitivity in 94.44 % of samples, resp. 96.3 %. In both methods, isolates were more often resistant than sensitive to most other antibiotics. We proved the presence of carbape- nemase in three isolates out of five selected. We found a statistically significant difference in the sensitivity to the antibiotics cefoperazone with sulbactam (p < 0.005) and piperacillin with tazobactam (p < 0.05), as to whether the isolates were derived from the airways (more sensitive) or urine (less sensitive) in the disc, diffusion test. We confirmed a statistically significant difference in the sensitivity to the antibiotics colistin (p < 0.05) and piperacillin with tazobactam (p < 0.05), based on whether the isolates were from the airway (more sensitive) or urine (less sensitive) in the microdilution method.\\\Conclusion: We confirmed the severity oi Klebsiella pneumoniae as a causative agent of nosocomial infections, due to the established resistance to several antibiotics.
- Publication
Zdravotnicke listy, 2021, Vol 9, Issue 4, p7
- ISSN
1339-3022
- Publication type
Article