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- Title
Sperm DNA fragmentation - can it be a routine?
- Authors
Natarajamani, Sasikala
- Abstract
Aim: To assess the correlation between semen parameters and sperm DNA fragmentation (SDF) and to examine whether SDF can be recommended as a routine test along with semen analysis. Materials and methods: Retrospective analysis was conducted for 118 male infertile patients attending a fertility clinic obtained from the records between 2017 and 2019. Semen analysis and SDF were assessed according to World Health Organization - 2010 guidelines and sperm chromatin dispersion test, respectively. Patients were grouped based on their SDF scores as "low DNA fragmentation group (LDF)" if SDF ≤ 18% and as "high DNA fragmentation group (HDF)" if SDF > 18%. Mann-Whitney and Kruskal-Wallis tests were used to find the difference in the mean of semen parameters between SDF groups. Statistical analysis was carried out using STATA Version-14. Results: The mean value of sperm concentration, motility, and normal morphology was higher in LDF compared to HDF. Correlation analysis showed patients' age to be positively associated with SDF (r=0.125; P=0.102). Sperm concentration, progressive motility, and normal morphology had a weak negative correlation with SDF. Around 63% and 69% of patients with normal morphology and normal motility respectively had high SDF. In the HDF category, nearly 42% had normal morphology, whereas a 66% demonstrated normal motility. The results of Kruskal-Wallis test for patients who had a treatment outcome (conception) showed that patients in LDF group had 1.5 times higher chance of "conception and live birth." Conclusion: Men with normal semen parameters can still have a high level of SDF. SDF along with semen analysis can help assess the reproductive potential of a male better.
- Subjects
INDIA; DNA analysis; AGE distribution; SEMEN analysis; RETROSPECTIVE studies; ACQUISITION of data; SPERM motility; INFERTILITY; MEDICAL records; SPERM count; SPERMATOZOA
- Publication
Fertility Science & Research, 2022, Vol 9, Issue 1, p29
- ISSN
2394-4285
- Publication type
Article
- DOI
10.4103/fsr.fsr_41_21