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- Title
Molecular characterization using SS R markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia.
- Authors
DUQUE-ZAPATA, JUAN DIEGO; EDUARDO MUÑOZ, JAIME; CHECA-CORAL, OSCAR
- Abstract
The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.
- Subjects
PEA genetics; PEA breeding; ALLELES in plants; GENETIC markers in plants
- Publication
Revista Colombiana de Ciencias Hortícolas, 2019, Vol 13, Issue 2, p208
- ISSN
2011-2173
- Publication type
Article
- DOI
10.17584/rcch.2019v13i2.10177