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- Title
Bacterial decontamination of toothbrushes by immersion in a mouthwash containing 0.05% chlorhexidine and 0.05% cetylpyridinium chloride: A randomized controlled trial.
- Authors
Àlvarez, Gerard; Soler‐Ollé, Agnès; Isabal, Sergio; León, Rubén; Blanc, Vanessa
- Abstract
Objective: Toothbrushes are colonized by microorganisms, implying a risk of infection. That risk can be reduced by decreasing the microbial contamination of the filaments. Therefore, this study aimed to determine the antiseptic efficacy of a 0.05% chlorhexidine + 0.05% cetylpyridinium chloride mouthwash on toothbrushes. Methods: A total of twelve toothbrushes used three times/day for 14 days by orally and systemically healthy people were randomly split into two groups, and their heads were immersed for 2 h in PBS (control) or Perio·Aid Active Control (treatment). The microorganisms were recovered, and their number was calculated by culture, quantitative PCR, and viability PCR. Statistical differences were first assessed with a two‐way mixed ANOVA and subsequently with Student's t‐test. Results: The results showed no statistical differences in the total number of cells for the treatment (mean ± CI95% of 7.27 ± 1.09 log10 bacteria/ml) and the control (7.62 ± 0.64 log10 bacteria/ml) groups, but a significantly lower number of live cells in the treatment group (4.58 ± 0.61 log10 viable bacteria/ml and 2.15 ± 1.42 log10 cfu/ml) than in the control group (6.49 ± 1.39 log10 viable bacteria/ml and 5.04 ± 0.93 log10 cfu/ml). Conclusions: Based on our findings, sanitization of toothbrushes with this mouthwash reduces the number of live microorganisms adhered to the filaments. Such decrease of the bacterial load could include bacteria from the oral cavity, from the environment, and from nearby toothbrushes since the quantification was not limited to any bacterial taxon.
- Subjects
BACTERIAL disease prevention; TOOTHBRUSHES; PYRIDINE; DRUG efficacy; STATISTICS; BACTERICIDES; ANALYSIS of variance; CONFIDENCE intervals; MEDICAL equipment contamination; BACTERIAL contamination; MOUTHWASHES; COLONY-forming units assay; RANDOMIZED controlled trials; COMPARATIVE studies; T-test (Statistics); DESCRIPTIVE statistics; BACTERIAL growth; MICROBIOLOGICAL techniques; RESEARCH funding; STERILIZATION (Disinfection); CHLORHEXIDINE; STATISTICAL sampling; POLYMERASE chain reaction; DATA analysis; MICROBIAL sensitivity tests; BACTERIA; PREVENTION; EVALUATION
- Publication
International Journal of Dental Hygiene, 2023, Vol 21, Issue 2, p357
- ISSN
1601-5029
- Publication type
Article
- DOI
10.1111/idh.12652