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- Title
FUNCTIONAL METATRANSCRIPTOMICS REVEALS A NOVEL MICRO-EUKARYOTIC GENE WITH POTENTIAL INVOLVEMENT IN METAL ION BINDING AND CATALYTIC ACTIVITIES.
- Authors
Yadav, Bhupendra Narayan Singh; Sharma, Priyanka; Maurya, Shristy; Yadav, Rajiv Kumar
- Abstract
The continuous introduction of heavy metal pollutants into the soil due to human activities can be harmful to both macro and microorganisms within the ecosystem. This study utilized a functional metatranscriptomics approach to investigate the potential functional roles of eukaryotic microbes in such environments. Micro-eukaryotic specific cDNA libraries of different sizes were created from high-quality RNA isolated from heavy metal contaminated soil. These libraries underwent screening for genes associated with cadmium (Cd) tolerance by functional complementation in a cadmium-sensitive yeast mutant strain. Screening of the cDNA libraries resulted in the isolation of various clones with the ability to thrive in a medium supplemented with cadmium. In this study, the transcript JMB-11 from one of such libraries was characterized and assessed for its capacity to withstand elevated levels of the heavy metal. JMB-11 sequence analysis showed homology to a hypothetical aldose 1-epimerase and demonstrated the ability to withstand high Cd concentration. The recognized protein, displaying distinctive traits of aldose 1-epimerase, not only clarifies its involvement in linking lactose and galactose metabolism but also underscores its importance as a metal ion-binding protein essential for regulating metal in polluted soil. This research establishes that JMB-11 serves as a newly identified and potent gene linked to metal tolerance. It holds potential applications in the regeneration of heavy metal contaminated soils or as a biomarker for detecting metal pollution.
- Subjects
CATALYTIC activity; HEAVY metals; METAL ions; GALACTOSE; METAL detectors; SEQUENCE analysis
- Publication
Biochemical & Cellular Archives, 2024, Vol 24, Issue 1, p51
- ISSN
0972-5075
- Publication type
Article
- DOI
10.51470/bca.2024.24.1.51