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- Title
四川省某奶牛场犊牛呼吸道疾病的细菌性病原qPCR检测.
- Authors
陈久兵; 耿尚景超; 王方国; 陈娅婷; 周金伟; 骆巧; 马莉; 姚学萍; 余树民; 沈留红; 储岳峰; 曹随忠
- Abstract
In order to explore the pathogens of bovine respiratory disease(BRD) in a large-scale dairy farm in Sichuan Province, TaqMan fluorescent quantitative PCR method was used to analyze 75 nasal cotton samples from the calves with respiratory disease symptoms and 10 other samples during 2019 to 2020 for common bacterial pathogens. The results showed that the detection rates of Pasteurella multocida(Pm), Histophilus somni(Hs), Mycoplasma bovis(Mb), Trueperella pyogenes(Tp) and Mannheimia haemolytica(Mh) were 57.33%(43/75), 38.67%(29/75), 13.33%(10/75), 12.00%(9/75), 8.00%(6/75). The single infection rate was 36.00% (27/75), mainly caused by Pm, accounting for 24.00%(18/75); the mixed infection rate was 40.00%(30/75), especially the mixed infection caused by Hs and Pm, accounted for 18.67%(14/75).The results showed that all five common BRD bacterial pathogens were detected, and there were many types of mixed infections. The high detection rate of Hs indicated that it had a certain connection with BRD in China, and it was necessary to carry out further epidemiological investigations nationwide.
- Subjects
SICHUAN Sheng (China); CHINA; MIXED infections; MANNHEIMIA haemolytica; SYMPTOMS; RESPIRATORY diseases; PASTEURELLA multocida; MYCOPLASMA bovis
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 4, p706
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.04.06