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- Title
Where Has My Efficiency Gone? Impacts of Extracolumn Peak Broadening on Performance, Part 4: Gradient Elution, Flow Splitting, and a Holistic View.
- Authors
Stoll, Dwight R.; Lauer, Thomas; Broeckhoven, Ken
- Abstract
Dispersion (broadening, or spreading) of analyte zones (peaks) outside of chromatography columns can seriously erode the resolution provided by good columns. In this final instalment of the series we focus on the impact of elution mode (isocratic or gradient) and postcolumn flow splitting on the total level of extracolumn dispersion (ECD) in a liquid chromatography (LC) system, and demonstrate the use of a free, web-based calculator that can be used to guide decision making aimed at reducing ECD during method development.
- Subjects
GRADIENT elution (Chromatography); LIQUID chromatography; COLUMN chromatography; DECISION making; DISPERSION (Chemistry)
- Publication
LC-GC Europe, 2021, Vol 34, Issue 11, p464
- ISSN
1471-6577
- Publication type
Article