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- Title
Fusarium wilt-resistant lines of Brazil banana ( Musa spp., AAA) obtained by EMS-induced mutation in a micro-cross-section cultural system.
- Authors
Chen, Y. F.; Chen, W.; Huang, X.; Hu, X.; Zhao, J. T.; Gong, Q.; Li, X. J.; Huang, X. L.
- Abstract
This study combined the micro-cross-section cultural system with in vitro mutagenesis induced by ethyl methanesulphonate (EMS) to screen for fusarium wilt-resistant lines of Brazil banana ( Musa spp., AAA). The results indicated that the optimum EMS concentration and duration for the treatment of micro-cross-sections cut from the pseudostem of tissue-cultured plantlet were 300 m m and 60 min, respectively. Under the optimal treatment, an average of 2·2 regenerated shoots were produced from each explant. One hundred regenerated plantlets were used for screening for fusarium wilt-resistant lines by the early screening technique. The initial disease symptom - yellowing in lower leaves of susceptible plantlets - was observed 2 weeks after inoculation. After 2 months, only six plants survived - the putative fusarium wilt-resistant lines. The fusarium wilt pathogen Fusarium oxysporum f. sp. cubense race 4, was identified in the preliminary test field by a SCAR marker technique. Of the six putative resistant lines, five survived the preliminary field test. The regenerated plantlets from these five fusarium wilt-resistant lines were subjected to early screening again, where they showed markedly reduced disease incidences compared with regenerated plantlets of Brazil banana (control). It was concluded that EMS-induced mutation of banana through the micro-cross-section cultural system is potentially useful for banana improvement.
- Subjects
BRAZIL; FUSARIUM oxysporum; MUTAGENESIS; BANANAS; PLANT mutation; ETHYL methanesulfonate
- Publication
Plant Pathology, 2013, Vol 62, Issue 1, p112
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.2012.02620.x