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- Title
A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis.
- Authors
Namba, Aki; Shigenobu, Yuya; Kobayashi, Masahiro; Kobayashi, Takanori; Oohara, Ichiro
- Abstract
To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were classified into four groups, namely, β- proteobacteria, γ- proteobacteria, Lentisphaerae, and Flavobacteria. PCR-based analysis of the 16S rDNA primer constructed in this study can be used to implement 16S rDNA-based methodologies for the investigation of microbial community composition and diversity related to the Porphyra group.
- Subjects
AQUACULTURE; DNA primers; PORPHYRA; PROKARYOTES
- Publication
Fisheries Science, 2010, Vol 76, Issue 5, p873
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1007/s12562-010-0273-z