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- Title
TheNeurospora crassagene responsible for thecutandovcphenotypes encodes a protein of the haloacid dehalogenase family.
- Authors
Youssar, Loubna; Schmidhauser, Thomas J.; Avalos, Javier
- Abstract
Light stimulation of carotenogenesis inNeurospora crassa, mediated by the White Collar proteins, is enhanced in some regulatory mutants, such asvividandovc. The gene responsible for thevividmutation has been identified, but not the one responsible for theovcphenotype. Theovcmutant is sensitive to high osmotic conditions and allelic with another mutant,cut, also osmosensitive but not affected in carotenogenesis. A phenotypic characterization of both strains is presented. Light induction of mRNA levels of the carotenoid genesal-1andal-2, the regulatory genewc-1or the conidiation-specific genecon-10is not significantly changed in theovcmutant when compared with the wild type. We have identified the gene affected in theovcmutant by complementation of osmosensitivity with a cosmid library. This gene, which we callcut-1, codes for an enzyme of the haloacid dehalogenase family, which includes different classes of phosphatases.cut-1is able to restore the wild-type phenotype of theovcandcutstrains, confirming that they are affected in the same gene. DNA sequence analysis identified a point mutation in the cut mutant, leading to a truncated protein. Theovcmutant represents a deletion encompassing the entire gene and surrounding sequences. Thecut-1promoter contains putative regulatory elements involved in osmotic or thermal stress. We show thatcut-1transcription is low in illuminated or dark-grown cultures, and is induced by high osmotic conditions or by heat shock.
- Subjects
PROTEINS; HALOACID dehalogenase; PHENOTYPES; GENETICS; BIOMOLECULES; GENES
- Publication
Molecular Microbiology, 2005, Vol 55, Issue 3, p828
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.2004.04424.x