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- Title
Screening of High-yielding Strain for the Extraction of Total Saponins from Agave sisalana perr. ex Engelm and Its Optimal Fermentation Condition.
- Authors
ZHOU, Feifei; HAO, Zaibin; LIAO, Dongmei; ZHANG, Fei; YI, Kexian; ZHENG, Jinlong; WANG, Xiuli
- Abstract
[Objective] To screen the high-yielding strain for the extraction of total saponins from Agave sisalana perr. ex Engelm and its optimal fermentation condition. [Methods] Hundreds of soil samples were collected from the nearby mountains of Guilin City. Strain JMZ-N06 was obtain based on screening. Total saponins were extracted from A. sisalana residues by using strain JMZ-N06. L9(34) orthogonal test was carried out with the factors of solid-liquid ratio, fermentation time, fermentation temperature and pH value of fermentation liquid. [Results] The optimal extraction technology was as follows; 1: 20 solid-liquid ratio (50 g residues in 1 L fermentation liquid), 60 h fermentation time, 32 °C fermentation temperature and pH 6.0 of fermentation liquid. [Conclusions] Stain JMZ-N06 and the extraction technology were both stable; and stain JMZ-N06 was high high-yielding strain for the extraction of total saponins from A. sisalana.
- Subjects
PHYSIOLOGIC strain; SAPONINS; SISAL (Plant); FERMENTATION; EXTRACTION (Chemistry); BIOENGINEERING
- Publication
Medicinal Plant, 2014, Vol 5, Issue 10, p40
- ISSN
2152-3924
- Publication type
Article