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- Title
VP16 serine 375 is a critical determinant of herpes simplex virus exit from latency in vivo.
- Authors
Sawtell, Nancy; Triezenberg, Steven; Thompson, Richard
- Abstract
Development of novel prevention and treatment strategies for herpes simplex virus (HSV) mediated diseases is dependent upon an accurate understanding of the central molecular events underlying the regulation of latency and reactivation. We have recently shown that the transactivation function of the virion protein VP16 is a critical determinant in the exit from latency in vivo. HSV-1 strain SJO2 carries a single serine to alanine substitution at position 375 in VP16 which disrupts its interaction with its essential co-activator Oct-1. Here we report that SJO2 is severely impaired in its ability to exit latency in vivo. This result reinforces our prior observations with VP16 transactivation mutant, in1814, in which VP16 interaction with Oct-1 is also disrupted and solidifies the importance of the VP16-Oct-1 interaction in the early steps in HSV-1 reactivation.
- Subjects
HERPES simplex prevention; SERINE; DRUG development; VIRAL proteins; GENETIC mutation; ALANINE; PHOSPHORYLATION; MOLECULAR genetics
- Publication
Journal of NeuroVirology, 2011, Vol 17, Issue 6, p546
- ISSN
1355-0284
- Publication type
Article
- DOI
10.1007/s13365-011-0065-y