We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differential DNA Methylation of Growth Factors in Antlers of Sika Deer and Reindeer.
- Authors
Jian-Cheng Zhai; Sheng-Nan Wang; Qiang-Hui Wang; Yan-Ling Xia; Wei-Shi Liu; Ya-Jie Yin; He-Ping Li
- Abstract
Reindeer is the only deer species in both sexes grow antlers. Many apparent differences exist in antlers of female and male reindeer and sika deer. Insulin like growth factor 1 (IGF1), Keratinocyte growth factor (KGF) and Nerve Growth Factor (NGF) are essential for antler growth. To investigate whether epigenetic regulation of the growth factors is important in growth of sika deer, female and male reindeer antlers, methylation status were evaluated using bisulfite sequencing PCR (BSP). The 5' flanking regions of IGF1, KGF and NGF were cloned from reindeer and sika deer antlers, and the lengths were 2089bp/2107bp, 1474bp/1474bp and 865bp/865bp, respectively. Based on bioinformatics analysis, the regions of IGF1 (-50bp ~ +615bp), KGF (-285bp ~ +207bp) and NGF (-388bp ~ +109bp) were selected for studying the methylation status. The antler mesenchyme of male reindeer was the only methylation sample in IGF1 (2.23 ± 0.92%), significantly higher in methylation level than that of female reindeer and sika deer (0.01<P<0.05). Methylation levels of KGF in antler mesenchyme were highly significantly higher in male reindeer and sika deer (45.53 ± 3.87% and 42.20 ± 1.91%, respectively), compared to female reindeer (0.00 ± 0.00%, P<0.01). Methylation level of NGF in antler mesenchyme was highly significantly higher in female reindeer (33.90 ± 3.57%), compared to male reindeer and sika deer (17.20 ± 3.57% and 21.57 ± 1.21%, respectively. P<0.01). We concluded that different methylation patterns of IGF1, KGF and NGF existed among antler mesenchyme of sika deer, female and male reindeer, and KGF might be an important candidate for regulating the unique growth of female reindeer antler.
- Subjects
SIKA deer; REINDEER; GROWTH factors; DNA methylation; KERATINOCYTE growth factors; METHYLGUANINE; NEUROTROPHINS
- Publication
Pakistan Journal of Zoology, 2023, Vol 55, Issue 3, p1175
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/20190718030701