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- Title
Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.
- Authors
Jain, Piyush K.; Ramanan, Vyas; Schepers, Arnout G.; Dalvie, Nisha S.; Panda, Apekshya; Fleming, Heather E.; Bhatia, Sangeeta N.
- Abstract
The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called 'CRISPR-plus' (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.
- Subjects
CRISPRS; PHOTOACTIVATION; NUCLEOTIDE sequencing; DIAGNOSTIC imaging; PHOTOCHEMISTRY
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 40, p12440
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201606123