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- Title
Effective PHIP Labeling of Bioactive Peptides Boosts the Intensity of the NMR Signal.
- Authors
Sauer, Grit; Nasu, Daichi; Tietze, Daniel; Gutmann, Torsten; Englert, Simon; Avrutina, Olga; Kolmar, Harald; Buntkowsky, Gerd
- Abstract
A series of novel bioactive derivatives of the sunflower trypsin inhibitor-1 (SFTI-1) suitable for hyperpolarization by parahydrogen-induced polarization (PHIP) was developed. The PHIP activity was achieved by labeling with L-propargylglycine, O-propargyl- L-tyrosine, or 4-pentynoic acid. 1H NMR signal enhancements (SE) of up to a factor of 70 were achieved in aqueous solution. We found that an isolated spatial location of the triple bond within the respective label and its accessibility for the hydrogenation catalyst are essential factors for the degree of signal enhancement.
- Subjects
PEPTIDES; POLARIZATION (Electrochemistry); PARAHYDROGEN; TRYPSIN inhibitors; HYPERPOLARIZATION (Cytology)
- Publication
Angewandte Chemie International Edition, 2014, Vol 53, Issue 47, p12941
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201404668