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- Title
The effect of continuously compressive press on the shape of cells and expressions of MMP-9,TRAP in human osteoclasts.
- Authors
XU Hai-yan; ZHOU Hong; RAO Guo-zhou
- Abstract
PURPOSE: To investigate the effect of continuously compressive pressure on the function of human osteoclasts. METHODS: Monocytes were collected from umbilical cord cells, and were cultured for 14 days. α-MEM which contains RANKL and M-CSF was used as the culture medium. 100kPa compressive pressure was applied to the mature osteoclasts for 1 hour, 2 hours and 3 hours. The shape change was observed. TRAP, MMP-9 enzymes were stained, and measured randomly. The data were analyzed with SPSS12.0 software package for Student's t test. RESULTS: Compressive pressure made the shape of osteoclasts incline to round. After adding pressure for 1 hour, there was significant difference on the expression of TRAP between pressure group(59.61±14.95)and non-pressure group(80.01±9.69) (P<0.05). Significant increase expression of MMP-9 occurred after 5 hours (P<0.05). CONCLUSION: The changes of shape and levels of functional enzymes show that compressive pressure enhances the bone-absorbing activity. Supported by National Natural Science Foundation of China (Grant No.30471911).
- Subjects
CELLS; OSTEOCLASTS; MONOCYTES; UMBILICAL cord; BONES
- Publication
Shanghai Journal of Stomatology, 2008, Vol 17, Issue 3, p285
- ISSN
1006-7248
- Publication type
Article