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- Title
Inexpensive fine mapping and positional cloning in plants using visible, mapped transgenes.
- Authors
Dinka, Stephen J.; Raizada, Manish N.
- Abstract
Vast numbers of crop, fungal, and animal accessions as well as insect vectors and evolving eukaryotic pathogens await molecular analysis. Inexpensive methods are required to make map-based gene isolation accessible to more of the world’s researchers. Today, positional cloning relies on genotyping and phenotyping large numbers of progeny to detect chromosome recombination events that break linkage between the trait of interest and flanking molecular markers following meiosis. In the postgenome era, positional cloning will no longer be limited by the availability of high-density molecular markers but rather by the skilled labour and the expense of genotyping and phenotyping 103-104 progeny to detect rare recombination events in a narrow chromosome block flanking the target gene of interest. Here, we review how linked, mapped transgenes that encode dominant, visible traits such as green fluorescent protein can be used to preselect meiotic recombinants inexpensively, thus reducing progeny genotyping and phenotyping requirements by >95% during positional cloning. Because transgene markers such as green fluorescent protein are genotype independent, transgenes created in one inbred line may be used to fine-map genetic variation in large numbers of genotypes.
- Subjects
PLANT gene mapping; PLANT clones; TRANSGENES; PLANT genetics; PATHOGENIC microorganisms; PHENOTYPES; CHROMOSOMES; MEIOSIS; GENES
- Publication
Canadian Journal of Botany, 2006, Vol 84, Issue 2, p179
- ISSN
0008-4026
- Publication type
Article
- DOI
10.1139/B05-170