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- Title
Human Epidermal Cells Synthesize HLA-DR Alloantigens In Vitro upon Stimulation with γ-Interferon.
- Authors
Volc-Platzer, Beatrix; Leibl, Heinz; Luger, Thomas; Zahn, Gabriele; Stingl, Georg
- Abstract
Under certain pathologic conditions, human keratinocytes synthesize and express HLA-DR antigens. Assuming that soluble mediators might be responsible for this phenomenon, differentiating, primarily DR- keratinocytes were grown in the presence or absence of mixed leukocyte culture supernatants and tested for Ia antigen expression. After 6 days of culture, keratinocytes displayed surface-bound HLA-DR α/β complexes when exposed to mixed leukocyte culture supernatants but not when cultured in media alone. These HLA-DR moieties on keratinocytes result from active biosynthesis as evidenced by the demonstration of the intracytoplasmic HLA-DR γ (invariant) chain within these cells. In view of reports that interferon-γ promotes Ia-production in a variety of cell types, we reasoned that this lymphokine might be responsible for the Ia-inducing property of mixed lymphocyte culture supernatants. Indeed, recombinant interferon-γ, but not interferon-α or interleukin-2, proved to be a potent stimulator of La expression by keratinocytes. The further finding that this event can be prevented by the addition of a monoclonal anti-interferon-γ antibody strongly suggests that this cytokine is directly responsible for HLA-DR production by keratinocytes. The interferon-γ-induced acquisition of HLA-DR antigens by primarily DR[sup-] keratinocytes may provide a useful tool to study the role of these alloantigens in T-cell activation and may also add to our understanding of mechanisms operative in epidermal cell-T-cell interactions.
- Subjects
EPIDERMIS; LANGERHANS cells; HLA histocompatibility antigens; INTERFERONS; HISTOCOMPATIBILITY antigens; LYMPHOKINES
- Publication
Journal of Investigative Dermatology, 1985, Vol 85, Issue 1, p16
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12274511