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- Title
Clinical utility of serial analysis of circulating tumour cells for detection of minimal residual disease of metastatic nasopharyngeal carcinoma.
- Authors
Ko, Josephine Mun-Yee; Vardhanabhuti, Vince; Ng, Wai-Tong; Lam, Ka-On; Ngan, Roger Kai-Cheong; Kwong, Dora Lai-Wan; Lee, Victor Ho-fun; Lui, Yun-Hoi; Yau, Chun-Chung; Kwan, Chung-Kong; Li, Wing-Sum; Yau, Stephen; Guo, Chen; Choi, Sheyne Sta Ana; Lei, Lisa Chan; Chan, Kenneth Chun-Ho; Lam, Candy Chi-Shan; Chan, Candy King-Chi; Dai, Wei; Khong, Pek-Lan
- Abstract
<bold>Background: </bold>Nasopharyngeal carcinoma (NPC) is an important cancer in Hong Kong. We aim to utilise liquid biopsies for serial monitoring of disseminated NPC in patients to compare with PET-CT imaging in detection of minimal residual disease.<bold>Method: </bold>Prospective serial monitoring of liquid biopsies was performed for 21 metastatic patients. Circulating tumour cell (CTC) enrichment and characterisation was performed using a sized-based microfluidics CTC chip, enumerating by immunofluorescence staining, and using target-capture sequencing to determine blood mutation load. PET-CT scans were used to monitor NPC patients throughout their treatment according to EORTC guidelines.<bold>Results: </bold>The longitudinal molecular analysis of CTCs by enumeration or NGS mutational profiling findings provide supplementary information to the plasma EBV assay for disease progression for good responders. Strikingly, post-treatment CTC findings detected positive findings in 75% (6/8) of metastatic NPC patients showing complete response by imaging, thereby demonstrating more sensitive CTC detection of minimal residual disease. Positive baseline, post-treatment CTC, and longitudinal change of CTCs significantly associated with poorer progression-free survival by the Kaplan-Meier analysis.<bold>Conclusions: </bold>We show the potential usefulness of application of serial analysis in metastatic NPC of liquid biopsy CTCs, as a novel more sensitive biomarker for minimal residual disease, when compared with imaging.
- Subjects
RESEARCH; CARCINOGENESIS; RESEARCH methodology; METASTASIS; EVALUATION research; MEDICAL cooperation; COMPARATIVE studies; KAPLAN-Meier estimator; METABOLISM
- Publication
British Journal of Cancer, 2020, Vol 123, Issue 1, p114
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/s41416-020-0871-1