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- Title
Influence of Complement Components on the Size and the Opsonization of DNA-Anti-DNA Complexes.
- Authors
Lamers, M. C.; de Groot, E. R.; Daha, M. R.; Roos, D.
- Abstract
We studied the influence of complement components on the size and the phagocytosis of DNA anti-DNA complexes. Large precipitating complexes, prepared with either IgG or IgM anti-DNA, were readily solubilized by fresh normal serum. This process can proceed via the alternative pathway of complement activation and is probably C3-dependent. When IgG anti-DNA DNA complexes were prepared in the presence of fresh normal serum, two phenomena were observed. Low concentrations of fresh serum (up to 2.5% v/v) led to an increase in the size of small IgG anti-DNA-DNA complexes. This increase in size was due to the interaction of Clq with the complexes. Higher concentrations of fresh serum prevented the formation of large complexes; this process consumes components of the classical pathway of complement activation and is most likely C3-dependent. The interaction both with Clq and with C3 needs the Fc parts of the IgG molecules. Phagocytosis of IgG anti-DNA-DNA complexes by monocytes and neutrophils from peripheral human blood was dependent on the size of the complexes rather than on fixation of C3, Fresh serum enhanced the phagocytosis of large complexes that were suboptimally opsonized with IgG anti-DNA. In contrast with the findings with IgG anti-DNA-DNA complexes, fresh normal serum did not detectably increase the site of IgM anti-DNA-DNA complexes; only prevention of the formation of large complexes was observed. Phagocytosis of IgM anti-DNA-DNA complexes was negligible in the absence of fresh serum. However, in the presence of fresh serum, phagocytosis by monocytes but not by neutrophils was observed. This process utilized the C3b receptor on the monocytes.
- Subjects
DNA; ANTIGEN-antibody reactions; PHAGOCYTOSIS; SERUM; NEUTROPHILS; IMMUNE response
- Publication
Scandinavian Journal of Immunology, 1982, Vol 16, Issue 5, p389
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1982.tb00739.x