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- Title
Proteomic study of sporadic inclusion body myositis.
- Authors
Ke Li; Chuanqiang Pu; Xusheng Huang; Jiexiao Liu; Yanling Mao; Xianghui Lu
- Abstract
Background Sporadic inclusion body myositis (s-IBM) is the most commonly occurring acquired inflammatory myopathy in elderly people (>45 years); however, pathogenic mechanisms are poorly understood and diagnostic tools are limited. In view of this, new therapeutic and diagnostic molecular markers for s-IBM need to be identified. Experimental design In this study, the proteomes from three s-IBM cases were compared with those from three cases of neurogenic muscular atrophy (control). Proteins were separated by 2-dimensional polyacrylamide gel electrophoresis and profiled by mass spectrometric sequencing and subsequently validated by western blot. Results Differential expression was noted in 29 proteins (16 upregulated and 13 downregulated) in s-IBM compared with the control group. Functions of these proteins include oxidative stress response, regulation of apoptosis, signal transduction, and cytoskeleton. Expression of both amyloid precursor protein (APP) and αB-crystallin was increased in s-IBM cases. Conclusions Our study reveals a unique pattern of protein expression in s-IBM, which should be further investigated in a wider cohort of IBM patients to fully realize the potential diagnostic or therapeutic benefits.
- Subjects
NONARTICULAR rheumatism; MYOSITIS; PATHOGENIC microorganisms; MUSCLE diseases; ATROPHY
- Publication
Proteome Science, 2014, Vol 12, Issue 1, p1
- ISSN
1477-5956
- Publication type
Article
- DOI
10.1186/s12953-014-0045-2